James Ainslie (9 June 1880 – 31 December 1953) was an Australian cricketer. He played nine first-class cricket matches for Victoria between 1900 and 1910.
| Format | Matches | Runs | Avg | 100s/50s | Top Score | Wickets | Bowl Avg | Best Bowling | Catches/St |
| First-class | 9 | 455 | 28.43 | 1/3 | 107* | 2 | 32.50 | 2/23 | 8/– |
